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Sunday, October 11, 2020 | History

2 edition of Automating the in vivo micronucleus assay in the mouse found in the catalog.

Automating the in vivo micronucleus assay in the mouse

Jan Grawe

Automating the in vivo micronucleus assay in the mouse

flow-cytometric assessment of genetic damage induced by lowlevels of ionizing radiation and by chemicals with different induction mechanisms

by Jan Grawe

  • 260 Want to read
  • 8 Currently reading

Published by Sveriges Lantbruksuniversitet in Uppsala .
Written in English

    Subjects:
  • Flow cytometry.,
  • Cytofluorometry.,
  • Cells -- Effect of radiation on.,
  • Genetic disorders.,
  • Mice -- Genetics.

  • Edition Notes

    Series of papers combined to form a thesis for the Department of Pathology, Swedish University of Agricultural Sciences, Uppsala.

    StatementJan Grawe.
    ContributionsSveriges Lantbruksuniversitet.
    The Physical Object
    Pagination[130p.] :
    Number of Pages130
    ID Numbers
    Open LibraryOL21736718M
    ISBN 109157646872

    BD® AbSeq Assay BD® Single-Cell Multiplexing Kit BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit Micronucleus Assays; Your Selections. Clear All. Product Type. Kits/Sets/Pairs (5) Reactivity. Mouse (1) Rat (1) Size. 25 Tests (2) . Protocol recommended for the short-term mouse peripheral blood micronucleus test. Mutagene (). (7) Hayashi, M. et al. In vivo Rodent Erythrocyte Micronucleus Assay. Mutation Research , (). (8) Higashikuni, N. and Sutou, S.

      Micronucleus Formation in Mouse Embryos. The preimplantation mouse embryo develops from a fertilized zygote to a to cell morula and then a to cell blastocyst without intervening cell growth over the course of ∼4 d, providing a .   Negative in the In-vivo micronucleus test in mice. (2) So, it's negative in everything but that's not enough. (3) The IARC working Group regards pulegone as non-genotoxic. (4) Carcinogenicity; Three-month rat and mouse studies - no precancerous cell formation. Two-year rat carcinogenicity study - no increase in liver tumors.

    Genotoxicity, Mouse Peripheral Blood Micronucleus Study. Categories. Biocompatibility Genotoxicity Since no single test can detect all genotoxic agents, a battery approach is usually taken to meet study and regulatory objective utilizing both mammalian and non-mammalian cell culture models. The Muta™Mouse assay and the Big Blue® mouse assay versus the mouse bone marrow micronucleus test 46 The mouse bone marrow micronucleus test: principles and procedures 61 Comparison of data from the mouse bone marrow micronucleus test and transgenic mouse test 61 Bone marrow 61 All organs


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Automating the in vivo micronucleus assay in the mouse by Jan Grawe Download PDF EPUB FB2

This assay is an in vivo cytogenetic test which uses erythrocytes in the bone marrow of rodents to detect chemical damage to the chromosomes or mitotic apparatus of mammalian cells. At the time of erythroblast development into a polychromatic erythrocyte (PCEs) in bone marrow, the main nucleus is extruded, so any micronucleus (MN) that has been Author: Abhishek Kumar Jain, Alok Kumar Pandey.

The rodent micronucleus assay has been most widely and frequently used as a representative in vivo assay system to assess mutagenicity of chemicals, regardless of endpoint of mutagenicity. The standard rodent micronucleus assay involves examination of IEs in bone marrow and sometimes blood.

Mammalian erythroblasts undergo maturation in the bone marrow, expelling the nucleus to form IEs but generally retain micronuclei. This cohort of IEs can be identified by various staining methods that depend on their high RNA content or the presence of the CD71 antigen on the Author: Laura Custer, Ann Doherty, Jeffrey C.

Bemis, Ray Proudlock. Introduction. The in vivo rodent erythrocyte micronucleus (MN) test is a widely accepted assay for assessing the potential of chemicals to induce cytogenetic damage (1,2).The assay evaluates the formation of MN-containing lagging chromosome fragments or whole chromosomes in polychromatic erythrocytes (PCEs, immature erythrocytes), as a consequence of chemical-induced Cited by: 3.

1 A high-throughput in vivo micronucleus assay for genome instability screening in mice Balmus Gabriel1,2, Karp Natasha A3, Ng Bee Ling4, Jackson Stephen P1,2, 5 Adams David J5* and McIntyre Rebecca E5* 1.

The Wellcome Trust/Cancer Research UK Gurdon Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QN, UK. Standard Test Procedures; Interpretation of Results; Screening Versions of the Test; Automation; References; Chapter 8.

The In Vivo Rodent Micronucleus Assay Abstract; Introduction; History; Fundamentals; Test Substance Considerations; Study Design; Manual Methods; Automated Analysis and Flow.

In vivo mouse micronucleus test. The mouse micronucleus test is a well-known mammalian cell test system that can be performed according to the corresponding ISO standard (ISO; Assad et al., a). Swiss Albino mice are first treated with the.

The micronucleus test is used in conjunction with the Comet assay, in vivo or in vitro, and it detects micronuclei, which are formed during mitosis when a chromosome is lagging behind and may be. Genotoxicity is commonly evaluated during the chemical safety assessment together with other toxicological endpoints.

The micronucleus test is always included in many genotoxic test guidelines for long time in many classes of chemicals, e.g., pharmaceutical chemicals, agricultural chemicals, food additives. Although the trend of the safety assessment of chemicals faces to animal.

validated: the in vitro metaphase chromosome aberration assay, the in vitro micronucleus assay (note 1) and the mouse lymphoma LY cell Tk (thymidine kinase) gene mutation assay (MLA). Conducted on a miniaturised format and using flow cytometry to score both micronuclei and cytotoxicity, the flow micronucleus assay provides a rapid prediction of the in vitro micronucleus test.

The miniaturised format enables the study to be performed using far less test item than an OECD study, and responsive delivery timelines. the micronucleus test as one of in vivo mammalian geno-toxicity tests.

Historical consideration of the micronucleus test The micronucleus was recognized in the end of the 19th century when Howell and Jolly found small inclusions in the blood taken from cats and rats. The small inclusions, called Howell-Jolly body, are also observed in the eryth.

Micronucleus Assay: The State of Art, and Future Directions. Multi- and unilamellar liposomal encapsulation of ciprofloxacin as ways to modify its phototoxicity and photodegradation.

Reprint of: A three-dimensional in vitro HepG2 cells liver spheroid model for genotoxicity studies. RENEB intercomparison exercises analyzing micronuclei.

An in vivo assay is also useful for further investigation of genotoxicity detected by an in vitro system. The mammalian in vivo micronucleus test is used for the detection of damage induced by the test chemical to the chromosomes or the mitotic apparatus of erythroblasts.

The test evaluates micronucleus. Mutation Research,(9) The Collaborative Study Group for the Micronucleus Test (). Micronucleus Test with Mouse Peripheral Blood Erythrocytes by Acridine Orange Supravital Staining: The Summary Report of the 5th Collaborative Study by CSGMT/JEMS.

MMS. Mutation Res., In vivo mouse bone marrow micronucleus test. The in vivo micronucleus test was performed according to OECD TG Five or seven male ICR mice (8 weeks old, ‐ g) per dose were orally administered with GHX02 at doses ofand mg/kg/day.

The rodent micronucleus assay has been widely used to evaluate chemical clastogenicity (Heddle et al. ; Mavournin et al. ) using bone marrow polychromatic erythrocytes as the target cells. Instead of bone marrow, mouse peripheral blood was introduced for use in the micronucleus assay.

In regulatory testing, the rodent erythrocyte MN assay is the most common first choice among in vivo assays used for subsequent testing when in vitro genotoxicity test(s) are found to be positive.

The unscheduled DNA synthesis assay using rat liver has also been used in this situation, but its sensitivity has been questioned (17). Details of the mammalian erythrocyte micronucleus assay are described by the OECD Guideline # from With Metafer evaluation of the assay can be fully automated.

Automation requires cellulose column purified samples according to the method described first. Proposal for updating Test Guideline In Vitro Mammalian Cell Micronucleus Test INTRODUCTION 1. The in vitro micronucleus (MNvit) assay is a genotoxicity test for the detection of micronuclei (MN) in the cytoplasm of interphase cells.

Micronuclei may originate from acentric chromosome. In vivo erythrocyte micronucleus assay: III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes with discussion of non-hematopoietic target cells and a single dose- level limit test.

Mutat Res. ; –  In vivoMicronucleus Assay Working Group, IWGT In vivo erythrocyte micronucleus assay III. Validation and regulatory acceptance of automated scoring and the use of rat peripheral blood reticulocytes, with discussion of non-hematopoietic target cells and a single dose-level limit test.

Mutat Res. ; –micronucleus formation if an acentric fragment is produced; therefore assays that detect either chromosomal aberrations or micronuclei are considered appropriate for detecting clastogens.

Micronuclei can also result from lagging of one or more whole chromosome(s) at anaphase and thus.